USU Conference Systems, International Conference on Tropical Medicine and Infectious Diseases (ICTROMI) 2017

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Pap smear cytology and identification of Human Papillomavirus (HPV) types 16 and 18 in multiparity women at Aviati Clinic Padang Bulan Medan
Dwi Rita Anggraini, Lita Feriyawati, Alya Amila Fitrie, Radita Nur Anggreini

Last modified: 2017-11-03

Abstract


Abstract. Cervical cancer is the second cancer in woman  in developing country and the most problem in the world. Human Papillomavirus (HPV) is an agent for sexual transmitted disease which is act in cervical cancer, specially high-risk of HPV type 16 and 18. In this study, we investigated the Pap smear cytology features and identification of HPV types 16 and 18  in multiparity women at Aviati Clinic Padang Bulan, Medan. Samples are cervical swabs of 50 multiparity women who met the inclusion criteria (childbirth ≥ 3 times) were included in the study. Pap smear examination were conducted using Papanicalaou staining and identification of HPV types 16 and 18 using the Polymerase Chain Reactive (PCR) methods. Pap smear  cytology showed 80% Negative for intraepithelial lesion or malignancy (NILM) with inflammation and 20% NILM with normal cells. The result of PCR amplification showed that there weren’t specific band DNA was found at band 414 bp and 216 bp. That means there weren’t  cervical swabs sample had DNA of HPV type 16 and 18.