It is common to see HBV infected couple seeking fertility treatment in reproductive medical centers. The effect of hepatitis B virus (HBV) infection on pregnancy outcome after In Vitro fertilization (IVF) treatment has been controversy. The aim of this study was to evaluate the outcome of in vitro fertilization in couple with male partner being HbsAg-seropositive. A retrospective analyzed study from secondary data of medical record was performed in HBV-infected male partner group and HBV-non infected group who have been treated with in vitro fertilization (IVF) from October 2016 until May 2017 in HFC IVF Center. From this study, we found 17 (16.83%) male partners were HBV seropositive in 101 couples. 17 male partners being HBV seropositive had similar semen parameters compared with HBV-non infected group. Couples with male partner being HbsAg-seropositive had significantly lower oocyte fertilized and cleaved embryos compared with HBV-non infected group. We also found lower clinical pregnancy rate in couples with male partner being HbsAg-seropositive compared with control group (23.52% vs 51%) respectively. Statistical analysis showed there was significant difference in clinical pregnancy rate between HBV-infected group and control group (p<0.05). HBV infected male partner may decrease clinical pregnancy rate in couple undergoing IVF treatment. The mechanism of HBV infection impact on IVF outcome needs further exploration.